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KMID : 0613820090190101396
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Journal of Life Science
2009 Volume.19 No. 10 p.1396 ~ p.1402
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Hizikia fusiforme Inhibits Cyclooxygenase-2 Expression and Prostaglandin E₂ Production by PMA through Inactivation of NF-kB
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Park Cheol
Choi Yung-Hyun
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Abstract
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Hizikia fusiforme is a kind of edible brown seaweed that grows mainly in the northwest Pacific including Korea, Japan and China, and has been widely used as food in Korea. Induction of cyclooxygenase-2 (COX-2) expression and prostaglandin E₂ (PGE₂) production is thought to have beneficial immunomodulatory effects in acute and chronic inflammatory disorders. In this study, we investigated the effects of extracts of H. fusiforme on the expression of COX-2 and production of PGE₂ in U937 human pre-monocytic cell models. In U937 cells stimulated with phorbol 12-myristate 13-acetate (PMA) to mimic inflammation, methanol extract of H. fusiforme (MEHF) and ethanol extract of H. fusiforme (EEHF), but not water extract of H. fusiforme (WEHF), inhibited PMA-induced expression of both COX-2 protein and mRNA, which was associated with inhibition of PGE₂ production. To investigate the mechanism by which MEHF and EEHF inhibit COX-2 gene expression and PGE₂ production, we examined the activation of nuclear factor-kappaB (NF-κB) in U937 cells. Pre-treatment with MEHF and EEHF significantly attenuated the PMA-induced IkappaB degradation and prevented nuclear translocation of NF-κB. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-inflammatory activity of H. fusiforme.
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KEYWORD
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Hizikia fusiforme, cyclooxygenase-2(COX-2), prostaglandin E₂(PGE₂), nuclear factor-kappaB(NF-κB)
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